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prolong gold antifade reagent mounting medium  (Thermo Fisher)


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    Thermo Fisher prolong gold antifade reagent mounting medium
    Prolong Gold Antifade Reagent Mounting Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prolong+gold+antifade+reagent+mounting+medium/prolong+gold+antifade+mounting+medium/pm40229314-89-10-11
    Average 90 stars, based on 1 article reviews
    prolong gold antifade reagent mounting medium - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Staining:

    Article Title: ID1 mediates escape from TGF-β tumor suppression in pancreatic cancer
    Article Snippet: Following incubation of the KLF5 goat polyclonal primary antibody, detection was performed using a rabbit anti-goat secondary (Vector lab, 1:4,000) followed by the aforementioned PerkinElmer Opal polymer. .. After 4 sequential rounds of staining, sections were stained with Hoechst 33342 (Invitrogen) to visualize nuclei and mounted with ProLong Gold antifade reagent mounting medium (Invitrogen). ..

    Article Title: Progressive plasticity during colorectal cancer metastasis
    Article Snippet: The covalent tyramide reaction was followed by heat-induced stripping of the primary–secondary antibody complex using Akoya AR9 buffer (AR900250ML) and Leica Bond ER2 (90% AR9 and 10% ER2) at 100 °C for 20 min preceding the next cycle (1 cycle of stripping for HER2 (1 μg ml −1 , CST, D8F12), CDX2 (0.46 μg ml −1 , CST, D11D10), TP63 (0.15 μg ml −1 , CST, D9L7L), PLCG2 (0.2 μg ml −1 , CST, E5U4T) and chromogranin A (2.705 μg ml −1 , Abcam, EP1030Y); 2 cycles for SOX2 (1.26 μg ml −1 , Abcam, SP76), CK20 (0.208 μg ml −1 , CST, D9Z1Z), VIM (0.0375 μg ml −1 , CST, D21H3), TROP2 (3.29 μg ml −1 , Abcam, SP294), CK5 (0.142 μg ml −1 , CST, E2T4B) and OLFM4 (0.27 μg ml −1 , CST, D1E4M); 2.5 cycles of stripping for Ki-67 (1:100, Biocare, SP6). .. After six sequential rounds of staining, the sections were stained with Hoechst (Invitrogen, 33342) to visualize nuclei and mounted with ProLong Gold antifade reagent mounting medium (Invitrogen, P36930). .. Seven-colour multiplex-stained slides were imaged using the Vectra Multispectral Imaging System version 3 (Akoya).

    Article Title: INaP selective inhibition reverts precocious inter- and motorneurons hyperexcitability in the Sod1-G93R zebrafish ALS model.
    Article Snippet: After rinsing with PBS-TT, the sections were incubated at RT for one hour with the secondary antibody: Alexa Fluor 488 F(ab’)2 Fragment of Goat Anti-Rabbit IgG (H + L) 1:200 and α -Bungarotoxin-Alexa Fluor 555 conjugate 1:500 (Molecular Probes) in blocking solution. .. After further rinsing with PBS-TT, the stained sections were mounted in ProLong Gold antifade reagent and mounting medium (Molecular Probes), and stored at 4 °C. ..

    Article Title: Immunologic signatures of response and resistance to nivolumab with ipilimumab in advanced metastatic cancer
    Article Snippet: The covalent tyramide reaction was followed by heat-induced stripping of the primary/secondary antibody complex using Akoya AR9 buffer and Leica Bond ER2 (90% AR9 and 10% ER2) at 100°C for 20 min preceding the next cycle. .. After six sequential rounds of staining, sections were stained with Hoechst 33342 (Invitrogen) to visualize nuclei and mounted with ProLong Gold antifade reagent mounting medium (Invitrogen). .. Seven-color multiplex stained slides were imaged using the Vectra Multispectral Imaging System version 3 (Akoya).

    Article Title: miR-145 Contributes to Hypertrophic Scarring of the Skin by Inducing Myofibroblast Activity.
    Article Snippet: Cells were stained with Texas Red–conjugated phalloidin (Life Technologies, [Thermo Fisher Scientific]) for 20 min at 37°C, washed with PBS and blocked with PBS containing 10% fetal calf serum for 40 min at room temperature. .. Subsequently, cells were stained with anti–α-SMA antibody for 1 h at room temperature, washed and incubated with Alexa Fluor 488–labeled secondary antibody (Life Technologies [Thermo Fisher Scientific]) for 1 h. The slides were mounted in ProLong Gold Antifade Reagent mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen [Thermo Fisher Scientific]). .. Fibroblasts were placed on an Olympus IX81 microscope (Olympus, Hamburg, Germany) with a fluorescein isothiocyanate (FITC), Texas Red or DAPI filter set using 10× and 40× objectives.

    Article Title: miR-145 Contributes to Hypertrophic Scarring of the Skin by Inducing Myofibroblast Activity
    Article Snippet: Cells were stained with Texas Red–conjugated phalloidin (Life Technologies, [Thermo Fisher Scientific]) for 20 min at 37°C, washed with PBS and blocked with PBS containing 10% fetal calf serum for 40 min at room temperature. .. Subsequently, cells were stained with anti–α-SMA antibody for 1 h at room temperature, washed and incubated with Alexa Fluor 488–labeled secondary antibody (Life Technologies [Thermo Fisher Scientific]) for 1 h. The slides were mounted in ProLong Gold Antifade Reagent mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen [Thermo Fisher Scientific]). .. Fibroblasts were placed on an Olympus IX81 microscope (Olympus, Hamburg, Germany) with a fluorescein isothiocyanate (FITC), Texas Red or DAPI filter set using 10× and 40× objectives.

    Incubation:

    Article Title: miR-145 Contributes to Hypertrophic Scarring of the Skin by Inducing Myofibroblast Activity.
    Article Snippet: Cells were stained with Texas Red–conjugated phalloidin (Life Technologies, [Thermo Fisher Scientific]) for 20 min at 37°C, washed with PBS and blocked with PBS containing 10% fetal calf serum for 40 min at room temperature. .. Subsequently, cells were stained with anti–α-SMA antibody for 1 h at room temperature, washed and incubated with Alexa Fluor 488–labeled secondary antibody (Life Technologies [Thermo Fisher Scientific]) for 1 h. The slides were mounted in ProLong Gold Antifade Reagent mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen [Thermo Fisher Scientific]). .. Fibroblasts were placed on an Olympus IX81 microscope (Olympus, Hamburg, Germany) with a fluorescein isothiocyanate (FITC), Texas Red or DAPI filter set using 10× and 40× objectives.

    Article Title: miR-145 Contributes to Hypertrophic Scarring of the Skin by Inducing Myofibroblast Activity
    Article Snippet: Cells were stained with Texas Red–conjugated phalloidin (Life Technologies, [Thermo Fisher Scientific]) for 20 min at 37°C, washed with PBS and blocked with PBS containing 10% fetal calf serum for 40 min at room temperature. .. Subsequently, cells were stained with anti–α-SMA antibody for 1 h at room temperature, washed and incubated with Alexa Fluor 488–labeled secondary antibody (Life Technologies [Thermo Fisher Scientific]) for 1 h. The slides were mounted in ProLong Gold Antifade Reagent mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen [Thermo Fisher Scientific]). .. Fibroblasts were placed on an Olympus IX81 microscope (Olympus, Hamburg, Germany) with a fluorescein isothiocyanate (FITC), Texas Red or DAPI filter set using 10× and 40× objectives.

    Microscopy:

    Article Title: Helicobacter pylori -induced Sonic Hedgehog expression is regulated by NFκB pathway activation: The use of a novel in vitro model to study epithelial response to infection
    Article Snippet: Sections were then incubated with a 1:100 dilution of goat anti-mouse Alexa Fluor 633 secondary antibody (Invitrogen, Carlasbad, CA) for 1 hour at room temperature. .. Coverslips were mounted onto slides with Prolong Gold Antifade Reagent Mounting Medium (Molecular Probes) and analyzed with a Zeiss LSM510 META confocal microscope. ..



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